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human metastatic melanoma cell lines mewo  (ATCC)


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    ATCC human metastatic melanoma cell lines mewo
    (A) Schematic representation of the zebrafish xenograft assay. (B) Migration capacity was assessed in <t>MeWo-GFP</t> or A375P-GFP cells. Starting 48h after siRNA transfection, cell migration was tracked by live microscopy for 16 h. Migration velocity was reduced in both cell lines following PFKFB4 knockdown with siRNA targeting PFKFB4 (siPFKFB4), compared with cells transfected with non-targeting control siRNA (siCtrl). Bar plots represent mean ± SEM of three independent experiments (unpaired t- test). Kernel density plots illustrate the distribution of migration speeds in siCtrl versus siPFKFB4 groups. (C) (E) Number of invasive MeWo-GFP foci (C) or A375P-GFP foci (E) per fish at 3 days post injection (dpi) after transfection with siCtrl or siPFKFB4. (D) (F) Representative zebrafish larvae at 3 dpi xenografted with MeWo-GFP (D) or A375P-GFP (F) cells after transfection with siCtrl or siPFKFB4. Asterisks indicate invasive foci detached from the primary tumor. Orange dotted lines mark the swim bladder boundary. (G) Number of distant <t>metastatic</t> A375P-GFP foci, defined as cells migrating > 300 μm from the tumor boundary. (H) Magnification of the tail region showing A375P-GFP cells metastasizing to caudal hematopoietic tissue. In panels C , E , and G , each dot represents one fish. Statistical significance was determined by unpaired t- test. n.s., not significant (P > 0.05); *P < 0.05; dpi, days post injection; dpf, days post fertilization.
    Human Metastatic Melanoma Cell Lines Mewo, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 610 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human metastatic melanoma cell lines mewo/product/ATCC
    Average 96 stars, based on 610 article reviews
    human metastatic melanoma cell lines mewo - by Bioz Stars, 2026-02
    96/100 stars

    Images

    1) Product Images from "Zebrafish Xenografts Reveal a Context-dependent Role of PFKFB4 in Melanoma Cell"

    Article Title: Zebrafish Xenografts Reveal a Context-dependent Role of PFKFB4 in Melanoma Cell

    Journal: bioRxiv

    doi: 10.1101/2025.09.06.674616

    (A) Schematic representation of the zebrafish xenograft assay. (B) Migration capacity was assessed in MeWo-GFP or A375P-GFP cells. Starting 48h after siRNA transfection, cell migration was tracked by live microscopy for 16 h. Migration velocity was reduced in both cell lines following PFKFB4 knockdown with siRNA targeting PFKFB4 (siPFKFB4), compared with cells transfected with non-targeting control siRNA (siCtrl). Bar plots represent mean ± SEM of three independent experiments (unpaired t- test). Kernel density plots illustrate the distribution of migration speeds in siCtrl versus siPFKFB4 groups. (C) (E) Number of invasive MeWo-GFP foci (C) or A375P-GFP foci (E) per fish at 3 days post injection (dpi) after transfection with siCtrl or siPFKFB4. (D) (F) Representative zebrafish larvae at 3 dpi xenografted with MeWo-GFP (D) or A375P-GFP (F) cells after transfection with siCtrl or siPFKFB4. Asterisks indicate invasive foci detached from the primary tumor. Orange dotted lines mark the swim bladder boundary. (G) Number of distant metastatic A375P-GFP foci, defined as cells migrating > 300 μm from the tumor boundary. (H) Magnification of the tail region showing A375P-GFP cells metastasizing to caudal hematopoietic tissue. In panels C , E , and G , each dot represents one fish. Statistical significance was determined by unpaired t- test. n.s., not significant (P > 0.05); *P < 0.05; dpi, days post injection; dpf, days post fertilization.
    Figure Legend Snippet: (A) Schematic representation of the zebrafish xenograft assay. (B) Migration capacity was assessed in MeWo-GFP or A375P-GFP cells. Starting 48h after siRNA transfection, cell migration was tracked by live microscopy for 16 h. Migration velocity was reduced in both cell lines following PFKFB4 knockdown with siRNA targeting PFKFB4 (siPFKFB4), compared with cells transfected with non-targeting control siRNA (siCtrl). Bar plots represent mean ± SEM of three independent experiments (unpaired t- test). Kernel density plots illustrate the distribution of migration speeds in siCtrl versus siPFKFB4 groups. (C) (E) Number of invasive MeWo-GFP foci (C) or A375P-GFP foci (E) per fish at 3 days post injection (dpi) after transfection with siCtrl or siPFKFB4. (D) (F) Representative zebrafish larvae at 3 dpi xenografted with MeWo-GFP (D) or A375P-GFP (F) cells after transfection with siCtrl or siPFKFB4. Asterisks indicate invasive foci detached from the primary tumor. Orange dotted lines mark the swim bladder boundary. (G) Number of distant metastatic A375P-GFP foci, defined as cells migrating > 300 μm from the tumor boundary. (H) Magnification of the tail region showing A375P-GFP cells metastasizing to caudal hematopoietic tissue. In panels C , E , and G , each dot represents one fish. Statistical significance was determined by unpaired t- test. n.s., not significant (P > 0.05); *P < 0.05; dpi, days post injection; dpf, days post fertilization.

    Techniques Used: Xenograft Assay, Migration, Transfection, Microscopy, Knockdown, Control, Injection

    (A) Protein expression levels of the EMT-TFs SNAIL2, SNAIL1, TWIST, ZEB2 and ZEB1 across different melanoma cell lines. (B) mRNA expression of EMT-TFs in MeWo cells assessed by qPCR 48h after transfection with siCtrl or siPFKFB4. Statistical analysis was performed using an unpaired t-test. (C, D) Normalized protein expression of EMT-TFs in MeWo (C) or A375M (D) cells, 48h after transfection with siCtrl or siPFKFB4. Each dot represents one biological replicate with paired values connected by lines. Statistical analysis was performed using a paired t- test. n.s, not significant (P > 0.05); *P < 0.05; **P < 0.01.
    Figure Legend Snippet: (A) Protein expression levels of the EMT-TFs SNAIL2, SNAIL1, TWIST, ZEB2 and ZEB1 across different melanoma cell lines. (B) mRNA expression of EMT-TFs in MeWo cells assessed by qPCR 48h after transfection with siCtrl or siPFKFB4. Statistical analysis was performed using an unpaired t-test. (C, D) Normalized protein expression of EMT-TFs in MeWo (C) or A375M (D) cells, 48h after transfection with siCtrl or siPFKFB4. Each dot represents one biological replicate with paired values connected by lines. Statistical analysis was performed using a paired t- test. n.s, not significant (P > 0.05); *P < 0.05; **P < 0.01.

    Techniques Used: Expressing, Transfection

    (A) Mean migration velocity of MeWo cells 48 h after transfection with siCtrl or siRNA targeting SNAIL2 (siSNAIL2). Kernel density plots illustrate the distribution of migration speeds in control versus SNAIL2 knockdown groups. (B,C) Expression of PFKFB4 and SNAIL2 proteins (B) and mean migration velocity (C) of MeWo cells transfected with siCtrl or siRNA targeting PFKFB4 (siPFKFB4), combined with empty pcDNA vector or a plasmid expressing Xenopus laevis SNAIL2 (xSNAIL2). Kernel density plots show the distribution of migration speeds across groups. xSNAIL2 failed to rescue the migration defect induced by PFKFB4 knockdown. (D) Mean migration velocity of MeWo cells after siCtrl or siPFKFB4 transfection, combined with empty pcDNA vector or a plasmid expressing Xenopus laevis PFKFB4 (xPFKFB4). Kernel density plots show the distribution of migration speeds. xPFKFB4 was able to rescue the migration defect caused by PFKFB4 knockdown. (E, F) Protein expression levels of PFKFB4 and SNAIL2 (E) and quantification from four biological replicates (F) in MeWo cells transfected with siCtrl or siPFKFB4, combined with empty pcDNA or xPFKFB4 plasmid. xPFKFB4 failed to rescue the decrease in SNAIL2 protein levels caused by PFKFB4 knockdown. In panels A , C , and D , bar plots represent mean ±SEM and were analyzed using an unpaired t- test. (F) Each dot represents one biological replicate, with paired values connected by lines; significance was assessed using a paired t- test. n.s., not significant (P > 0.05); *P < 0.05.
    Figure Legend Snippet: (A) Mean migration velocity of MeWo cells 48 h after transfection with siCtrl or siRNA targeting SNAIL2 (siSNAIL2). Kernel density plots illustrate the distribution of migration speeds in control versus SNAIL2 knockdown groups. (B,C) Expression of PFKFB4 and SNAIL2 proteins (B) and mean migration velocity (C) of MeWo cells transfected with siCtrl or siRNA targeting PFKFB4 (siPFKFB4), combined with empty pcDNA vector or a plasmid expressing Xenopus laevis SNAIL2 (xSNAIL2). Kernel density plots show the distribution of migration speeds across groups. xSNAIL2 failed to rescue the migration defect induced by PFKFB4 knockdown. (D) Mean migration velocity of MeWo cells after siCtrl or siPFKFB4 transfection, combined with empty pcDNA vector or a plasmid expressing Xenopus laevis PFKFB4 (xPFKFB4). Kernel density plots show the distribution of migration speeds. xPFKFB4 was able to rescue the migration defect caused by PFKFB4 knockdown. (E, F) Protein expression levels of PFKFB4 and SNAIL2 (E) and quantification from four biological replicates (F) in MeWo cells transfected with siCtrl or siPFKFB4, combined with empty pcDNA or xPFKFB4 plasmid. xPFKFB4 failed to rescue the decrease in SNAIL2 protein levels caused by PFKFB4 knockdown. In panels A , C , and D , bar plots represent mean ±SEM and were analyzed using an unpaired t- test. (F) Each dot represents one biological replicate, with paired values connected by lines; significance was assessed using a paired t- test. n.s., not significant (P > 0.05); *P < 0.05.

    Techniques Used: Migration, Transfection, Control, Knockdown, Expressing, Plasmid Preparation



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    human metastatic melanoma cell lines mewo  (ATCC)
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    ATCC human metastatic melanoma cell lines mewo
    (A) Schematic representation of the zebrafish xenograft assay. (B) Migration capacity was assessed in <t>MeWo-GFP</t> or A375P-GFP cells. Starting 48h after siRNA transfection, cell migration was tracked by live microscopy for 16 h. Migration velocity was reduced in both cell lines following PFKFB4 knockdown with siRNA targeting PFKFB4 (siPFKFB4), compared with cells transfected with non-targeting control siRNA (siCtrl). Bar plots represent mean ± SEM of three independent experiments (unpaired t- test). Kernel density plots illustrate the distribution of migration speeds in siCtrl versus siPFKFB4 groups. (C) (E) Number of invasive MeWo-GFP foci (C) or A375P-GFP foci (E) per fish at 3 days post injection (dpi) after transfection with siCtrl or siPFKFB4. (D) (F) Representative zebrafish larvae at 3 dpi xenografted with MeWo-GFP (D) or A375P-GFP (F) cells after transfection with siCtrl or siPFKFB4. Asterisks indicate invasive foci detached from the primary tumor. Orange dotted lines mark the swim bladder boundary. (G) Number of distant <t>metastatic</t> A375P-GFP foci, defined as cells migrating > 300 μm from the tumor boundary. (H) Magnification of the tail region showing A375P-GFP cells metastasizing to caudal hematopoietic tissue. In panels C , E , and G , each dot represents one fish. Statistical significance was determined by unpaired t- test. n.s., not significant (P > 0.05); *P < 0.05; dpi, days post injection; dpf, days post fertilization.
    Human Metastatic Melanoma Cell Lines Mewo, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human metastatic melanoma cell lines mewo/product/ATCC
    Average 96 stars, based on 1 article reviews
    human metastatic melanoma cell lines mewo - by Bioz Stars, 2026-02
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    human lung metastatic melanoma mewo cell line  (ATCC)
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    (A) Schematic representation of the zebrafish xenograft assay. (B) Migration capacity was assessed in <t>MeWo-GFP</t> or A375P-GFP cells. Starting 48h after siRNA transfection, cell migration was tracked by live microscopy for 16 h. Migration velocity was reduced in both cell lines following PFKFB4 knockdown with siRNA targeting PFKFB4 (siPFKFB4), compared with cells transfected with non-targeting control siRNA (siCtrl). Bar plots represent mean ± SEM of three independent experiments (unpaired t- test). Kernel density plots illustrate the distribution of migration speeds in siCtrl versus siPFKFB4 groups. (C) (E) Number of invasive MeWo-GFP foci (C) or A375P-GFP foci (E) per fish at 3 days post injection (dpi) after transfection with siCtrl or siPFKFB4. (D) (F) Representative zebrafish larvae at 3 dpi xenografted with MeWo-GFP (D) or A375P-GFP (F) cells after transfection with siCtrl or siPFKFB4. Asterisks indicate invasive foci detached from the primary tumor. Orange dotted lines mark the swim bladder boundary. (G) Number of distant <t>metastatic</t> A375P-GFP foci, defined as cells migrating > 300 μm from the tumor boundary. (H) Magnification of the tail region showing A375P-GFP cells metastasizing to caudal hematopoietic tissue. In panels C , E , and G , each dot represents one fish. Statistical significance was determined by unpaired t- test. n.s., not significant (P > 0.05); *P < 0.05; dpi, days post injection; dpf, days post fertilization.
    Human Lung Metastatic Melanoma Mewo Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human lung metastatic melanoma mewo cell line/product/ATCC
    Average 96 stars, based on 1 article reviews
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    metastatic human melanoma cell lines mewo  (ATCC)
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    ATCC metastatic human melanoma cell lines mewo
    (A) Schematic representation of the zebrafish xenograft assay. (B) Migration capacity was assessed in <t>MeWo-GFP</t> or A375P-GFP cells. Starting 48h after siRNA transfection, cell migration was tracked by live microscopy for 16 h. Migration velocity was reduced in both cell lines following PFKFB4 knockdown with siRNA targeting PFKFB4 (siPFKFB4), compared with cells transfected with non-targeting control siRNA (siCtrl). Bar plots represent mean ± SEM of three independent experiments (unpaired t- test). Kernel density plots illustrate the distribution of migration speeds in siCtrl versus siPFKFB4 groups. (C) (E) Number of invasive MeWo-GFP foci (C) or A375P-GFP foci (E) per fish at 3 days post injection (dpi) after transfection with siCtrl or siPFKFB4. (D) (F) Representative zebrafish larvae at 3 dpi xenografted with MeWo-GFP (D) or A375P-GFP (F) cells after transfection with siCtrl or siPFKFB4. Asterisks indicate invasive foci detached from the primary tumor. Orange dotted lines mark the swim bladder boundary. (G) Number of distant <t>metastatic</t> A375P-GFP foci, defined as cells migrating > 300 μm from the tumor boundary. (H) Magnification of the tail region showing A375P-GFP cells metastasizing to caudal hematopoietic tissue. In panels C , E , and G , each dot represents one fish. Statistical significance was determined by unpaired t- test. n.s., not significant (P > 0.05); *P < 0.05; dpi, days post injection; dpf, days post fertilization.
    Metastatic Human Melanoma Cell Lines Mewo, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/metastatic human melanoma cell lines mewo/product/ATCC
    Average 96 stars, based on 1 article reviews
    metastatic human melanoma cell lines mewo - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Schematic representation of the zebrafish xenograft assay. (B) Migration capacity was assessed in MeWo-GFP or A375P-GFP cells. Starting 48h after siRNA transfection, cell migration was tracked by live microscopy for 16 h. Migration velocity was reduced in both cell lines following PFKFB4 knockdown with siRNA targeting PFKFB4 (siPFKFB4), compared with cells transfected with non-targeting control siRNA (siCtrl). Bar plots represent mean ± SEM of three independent experiments (unpaired t- test). Kernel density plots illustrate the distribution of migration speeds in siCtrl versus siPFKFB4 groups. (C) (E) Number of invasive MeWo-GFP foci (C) or A375P-GFP foci (E) per fish at 3 days post injection (dpi) after transfection with siCtrl or siPFKFB4. (D) (F) Representative zebrafish larvae at 3 dpi xenografted with MeWo-GFP (D) or A375P-GFP (F) cells after transfection with siCtrl or siPFKFB4. Asterisks indicate invasive foci detached from the primary tumor. Orange dotted lines mark the swim bladder boundary. (G) Number of distant metastatic A375P-GFP foci, defined as cells migrating > 300 μm from the tumor boundary. (H) Magnification of the tail region showing A375P-GFP cells metastasizing to caudal hematopoietic tissue. In panels C , E , and G , each dot represents one fish. Statistical significance was determined by unpaired t- test. n.s., not significant (P > 0.05); *P < 0.05; dpi, days post injection; dpf, days post fertilization.

    Journal: bioRxiv

    Article Title: Zebrafish Xenografts Reveal a Context-dependent Role of PFKFB4 in Melanoma Cell

    doi: 10.1101/2025.09.06.674616

    Figure Lengend Snippet: (A) Schematic representation of the zebrafish xenograft assay. (B) Migration capacity was assessed in MeWo-GFP or A375P-GFP cells. Starting 48h after siRNA transfection, cell migration was tracked by live microscopy for 16 h. Migration velocity was reduced in both cell lines following PFKFB4 knockdown with siRNA targeting PFKFB4 (siPFKFB4), compared with cells transfected with non-targeting control siRNA (siCtrl). Bar plots represent mean ± SEM of three independent experiments (unpaired t- test). Kernel density plots illustrate the distribution of migration speeds in siCtrl versus siPFKFB4 groups. (C) (E) Number of invasive MeWo-GFP foci (C) or A375P-GFP foci (E) per fish at 3 days post injection (dpi) after transfection with siCtrl or siPFKFB4. (D) (F) Representative zebrafish larvae at 3 dpi xenografted with MeWo-GFP (D) or A375P-GFP (F) cells after transfection with siCtrl or siPFKFB4. Asterisks indicate invasive foci detached from the primary tumor. Orange dotted lines mark the swim bladder boundary. (G) Number of distant metastatic A375P-GFP foci, defined as cells migrating > 300 μm from the tumor boundary. (H) Magnification of the tail region showing A375P-GFP cells metastasizing to caudal hematopoietic tissue. In panels C , E , and G , each dot represents one fish. Statistical significance was determined by unpaired t- test. n.s., not significant (P > 0.05); *P < 0.05; dpi, days post injection; dpf, days post fertilization.

    Article Snippet: Human metastatic melanoma cell lines MeWo (ATCC #HTB-65) , A375M , and A375P-GFP (ATCC #CRL-3224), as well as HEK-293FT cells (Thermo Fisher), were maintained in complete RPMI-1640 medium (MeWo, A375M; Gibco, #21875-034) or in complete DMEM (A375P, HEK-293FT; Gibco, # 41965-039), supplemented with 10% fetal bovine serum (Eurobio, #CVFSVF00-0U) and 1% penicillin/streptomycin (Sigma, #P0781).

    Techniques: Xenograft Assay, Migration, Transfection, Microscopy, Knockdown, Control, Injection

    (A) Protein expression levels of the EMT-TFs SNAIL2, SNAIL1, TWIST, ZEB2 and ZEB1 across different melanoma cell lines. (B) mRNA expression of EMT-TFs in MeWo cells assessed by qPCR 48h after transfection with siCtrl or siPFKFB4. Statistical analysis was performed using an unpaired t-test. (C, D) Normalized protein expression of EMT-TFs in MeWo (C) or A375M (D) cells, 48h after transfection with siCtrl or siPFKFB4. Each dot represents one biological replicate with paired values connected by lines. Statistical analysis was performed using a paired t- test. n.s, not significant (P > 0.05); *P < 0.05; **P < 0.01.

    Journal: bioRxiv

    Article Title: Zebrafish Xenografts Reveal a Context-dependent Role of PFKFB4 in Melanoma Cell

    doi: 10.1101/2025.09.06.674616

    Figure Lengend Snippet: (A) Protein expression levels of the EMT-TFs SNAIL2, SNAIL1, TWIST, ZEB2 and ZEB1 across different melanoma cell lines. (B) mRNA expression of EMT-TFs in MeWo cells assessed by qPCR 48h after transfection with siCtrl or siPFKFB4. Statistical analysis was performed using an unpaired t-test. (C, D) Normalized protein expression of EMT-TFs in MeWo (C) or A375M (D) cells, 48h after transfection with siCtrl or siPFKFB4. Each dot represents one biological replicate with paired values connected by lines. Statistical analysis was performed using a paired t- test. n.s, not significant (P > 0.05); *P < 0.05; **P < 0.01.

    Article Snippet: Human metastatic melanoma cell lines MeWo (ATCC #HTB-65) , A375M , and A375P-GFP (ATCC #CRL-3224), as well as HEK-293FT cells (Thermo Fisher), were maintained in complete RPMI-1640 medium (MeWo, A375M; Gibco, #21875-034) or in complete DMEM (A375P, HEK-293FT; Gibco, # 41965-039), supplemented with 10% fetal bovine serum (Eurobio, #CVFSVF00-0U) and 1% penicillin/streptomycin (Sigma, #P0781).

    Techniques: Expressing, Transfection

    (A) Mean migration velocity of MeWo cells 48 h after transfection with siCtrl or siRNA targeting SNAIL2 (siSNAIL2). Kernel density plots illustrate the distribution of migration speeds in control versus SNAIL2 knockdown groups. (B,C) Expression of PFKFB4 and SNAIL2 proteins (B) and mean migration velocity (C) of MeWo cells transfected with siCtrl or siRNA targeting PFKFB4 (siPFKFB4), combined with empty pcDNA vector or a plasmid expressing Xenopus laevis SNAIL2 (xSNAIL2). Kernel density plots show the distribution of migration speeds across groups. xSNAIL2 failed to rescue the migration defect induced by PFKFB4 knockdown. (D) Mean migration velocity of MeWo cells after siCtrl or siPFKFB4 transfection, combined with empty pcDNA vector or a plasmid expressing Xenopus laevis PFKFB4 (xPFKFB4). Kernel density plots show the distribution of migration speeds. xPFKFB4 was able to rescue the migration defect caused by PFKFB4 knockdown. (E, F) Protein expression levels of PFKFB4 and SNAIL2 (E) and quantification from four biological replicates (F) in MeWo cells transfected with siCtrl or siPFKFB4, combined with empty pcDNA or xPFKFB4 plasmid. xPFKFB4 failed to rescue the decrease in SNAIL2 protein levels caused by PFKFB4 knockdown. In panels A , C , and D , bar plots represent mean ±SEM and were analyzed using an unpaired t- test. (F) Each dot represents one biological replicate, with paired values connected by lines; significance was assessed using a paired t- test. n.s., not significant (P > 0.05); *P < 0.05.

    Journal: bioRxiv

    Article Title: Zebrafish Xenografts Reveal a Context-dependent Role of PFKFB4 in Melanoma Cell

    doi: 10.1101/2025.09.06.674616

    Figure Lengend Snippet: (A) Mean migration velocity of MeWo cells 48 h after transfection with siCtrl or siRNA targeting SNAIL2 (siSNAIL2). Kernel density plots illustrate the distribution of migration speeds in control versus SNAIL2 knockdown groups. (B,C) Expression of PFKFB4 and SNAIL2 proteins (B) and mean migration velocity (C) of MeWo cells transfected with siCtrl or siRNA targeting PFKFB4 (siPFKFB4), combined with empty pcDNA vector or a plasmid expressing Xenopus laevis SNAIL2 (xSNAIL2). Kernel density plots show the distribution of migration speeds across groups. xSNAIL2 failed to rescue the migration defect induced by PFKFB4 knockdown. (D) Mean migration velocity of MeWo cells after siCtrl or siPFKFB4 transfection, combined with empty pcDNA vector or a plasmid expressing Xenopus laevis PFKFB4 (xPFKFB4). Kernel density plots show the distribution of migration speeds. xPFKFB4 was able to rescue the migration defect caused by PFKFB4 knockdown. (E, F) Protein expression levels of PFKFB4 and SNAIL2 (E) and quantification from four biological replicates (F) in MeWo cells transfected with siCtrl or siPFKFB4, combined with empty pcDNA or xPFKFB4 plasmid. xPFKFB4 failed to rescue the decrease in SNAIL2 protein levels caused by PFKFB4 knockdown. In panels A , C , and D , bar plots represent mean ±SEM and were analyzed using an unpaired t- test. (F) Each dot represents one biological replicate, with paired values connected by lines; significance was assessed using a paired t- test. n.s., not significant (P > 0.05); *P < 0.05.

    Article Snippet: Human metastatic melanoma cell lines MeWo (ATCC #HTB-65) , A375M , and A375P-GFP (ATCC #CRL-3224), as well as HEK-293FT cells (Thermo Fisher), were maintained in complete RPMI-1640 medium (MeWo, A375M; Gibco, #21875-034) or in complete DMEM (A375P, HEK-293FT; Gibco, # 41965-039), supplemented with 10% fetal bovine serum (Eurobio, #CVFSVF00-0U) and 1% penicillin/streptomycin (Sigma, #P0781).

    Techniques: Migration, Transfection, Control, Knockdown, Expressing, Plasmid Preparation